Composite

Part:BBa_K2486014:Design

Designed by: Cau� Westmann, Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)


PdtxR reporter circuit (GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


Design Notes

We developed a new synthetic promoter that responds to DtxR, which possiblites a enhanced control of transcription, once DtxR doesn't interact with endogenous regulatory sites from E. coli<i> and <i>P. Agglomerans<i>.

Source

This part was generated through synthesis and posterior inserion in pSB1C3 through 3A assembly.

===References===